Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:glucose: 3.15 g/L;L-glutamine: yes;HEPES: no;sodium pyruvate: 0.055 g/L;phenol red: yes;NaHCO3: yes.

General description:Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application:Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites

to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment

to culture mouse squamous cell carcinoma (SCCVII) tumor cells